prime script rt reagent kit (TaKaRa)

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TaKaRa prime script rt reagent kit
Prime Script Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 88448 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prime+script+rt+kit/PrimeScript+RT+Reagent+Kit/pmc13101298-80-6-11
Average 99 stars, based on 88448 article reviews

prime script rt reagent kit - by Bioz Stars, 2026-09

99/100 stars

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Article Title: RNA-m5C regulatory atlas of human fetal tissues uncover the activities of Nsun2/Jarid2/Alyref axis
Article Snippet: RNA concentration was quantified using the Qubit RNA HS analysis kit (Thermo Fisher Scientific, Q32855 ). .. Total RNA was reverse transcribed into cDNA using the Prime Script RT kit (Takara, RR036A), followed by qRT-PCR using a 2 × SYBR Green qPCR master mix (BioSharp, BL698A). ..

Article Title: The robust, high-throughput, and temporally regulated roxCre and loxCre reporting systems for genetic modifications in vivo
Article Snippet: Cells were lysed with TRIzol (Invitrogen, 15596018), and total RNA was extracted according to the manufacturer’s instructions. .. For each sample, 1 μg of total RNA was reverse-transcribed into cDNA using the Prime Script RT kit (Takara, RR047A). .. The SYBR Green qPCR master mix (Thermo Fisher Scientific, 4367659) was used, and quantitative RT-PCR was performed on the QuantStudio 6 Real-Time PCR System (Thermo Fisher Scientific).

Article Title: Integrative analysis of non-small cell lung cancer identifies Jumonji domain-containing 6/ETS homologous factor axis as a target to overcome radioresistance
Article Snippet: .. Prime Script RT Kit (Takara, RR047A) was used for reverse transcription to cDNA. .. Quantitative PCR analysis was performed on the CFX Connect Real-Time PCR system (Bio-Rad, USA) using SsoFast EvaGreen Supermix (Bio-Rad, USA, 1725201).

Article Title: Selectively targeting BCL6 using a small-molecule inhibitor is a potential therapeutic strategy for glioblastoma
Article Snippet: .. RNA was extracted from U87-MG, U251, SF268, and HS683 cells treated with compounds for 24 h and reverse-transcribed into cDNA using Prime Script RT Kit (Takara). .. The cDNA was then used as a template for quantitative reverse transcription PCR reactions, and PCR reactions were performed with SYBR-Green (Takara).

Article Title: p38MAPK protects against myocardial ischemia-reperfusion injury by regulating GLUT4 expression and translocation through MEF2A/MEF2C in isolated rat heart.
Article Snippet: Aims: Myocardial ischemia-reperfusion injury (MIRI) remains a critical complication associated with cardiopulmonary bypass (CPB).. This study investigated whether p38 mitogen-activated protein kinase (p38MAPK) sustains myocardial glucose uptake by controlling glucose transporter-4 (GLUT4) expression and membrane translocation through distinct myocyte enhancer factor-2 (MEF2) isoforms.. Materials and Methods: MIRI was induced in Langendorff-perfused isolated rat hearts and oxygen-glucose deprivation/reoxygenation (OGD/R)-treated neonatal rat ventricular myocytes (NRVMs) and H9C2 cells. p38MAPK was inhibited with SB203580, MEF2 isoforms were overexpressed via lentiviral transduction. and GLUT4 translocation was assessed by subcellular fractionation, Wheat Germ Agglutinin (WGA) co-staining.

Quantitative RT-PCR:

SYBR Green Assay:

Real-time Polymerase Chain Reaction:

Synthesized:

Article Title: Chinese traditional medicine complex hemorrhoidal analgesic cream promotes wound healing in diabetic rats after anal fistula surgery via Keap1/Nrf2 signaling pathway
Article Snippet: Signals were developed using ECL chemiluminescence reagent (Millipore WBKLS050), and band intensities were quantified by Image Lab 6.0. .. Total RNA from wound tissue was extracted by the TRIzol method (Invitrogen, item no. 15596026), and cDNA was synthesized by the Prime Script RT kit (Takara, item no. RR047A). ..

Article Title: The KLKB1-TFE3-BRAF/MEK/ERK axis regulates neuronal ferroptosis in vascular dementia.
Article Snippet: .. Total RNA was extracted from hippocampal tissue or cells using TRIzol reagent (12183–555; Invitrogen), and complementary DNA (cDNA) was synthesized with a Prime Script RT kit (RR036A; TAKARA, Tokyo, Japan). .. Quantitative PCR was conducted on a StepOnePlus Real- Time PCR system (Stepone software v2.3; ABI, New York, USA) using TB Green Premix Ex Taq II (RR820A; TAKARA).

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